Научная статья на тему 'Both Na +/k +-pump and Na +,k +, 2Cl – cotransport contribute to cell volume control in human lung fibroblasts'

Both Na +/k +-pump and Na +,k +, 2Cl – cotransport contribute to cell volume control in human lung fibroblasts Текст научной статьи по специальности «Фундаментальная медицина»

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Аннотация научной статьи по фундаментальной медицине, автор научной работы — Koltsova S. V., Akimova O. A., Orlov S. N., Dulin N. O.

This study examined the relative impact of Na +/K +-pump and Na +,K +,2Cl – cotransport in the regulation of the volume of human lung fibroblasts. Inhibition of the Na +/K +-pump by ouabain or of Na +,K +,2Cl – cotransport by bumetanide for 24 hours attenuated the rate of K + ( 86Rb) influx in human lung fibroblasts from (1537 ± 124) cpm/well in control cells to (695 ± 14) and (522 ± 14) cpm/well in ouabainor bumetanide-treated cells, respectively, whereas simultaneous addition of both inhibitors decreased the rate of K + influx to (180 ± 8) cpm/well. Inhibition of either the Na +/K +-pump or of Na +,K +,2Cl – cotransport did not significantly change the volume of human lung fibroblasts, measured as 14C-urea available space, whereas simultaneous blockade of both ion transporters resulted in a 2-fold elevation of cell volume (table). Thus, our results demonstrate for the first time that both Na +/K +-pump and Na +,K +,2Cl – cotransport contribute to cell volume control in human lung fibroblasts. Effect of ouabain and bumetanide on the volume of human lung fibroblasts + Cell volume ml per mg protein pl per cell None (control) 0.79 ± 0.12 0.23 ± 0.04 Ouabain, 100 nM 0.97 ± 0.14 0.26 ± 0.04 Bumetanide, 10 mM 0.62 ± 0.01 0.22 ± 0.01 Ouabain + bumetanide 1.37 ± 0.13* 0.43 ± 0.04* * p < 0.05 as compare to controls.

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Текст научной работы на тему «Both Na +/k +-pump and Na +,k +, 2Cl – cotransport contribute to cell volume control in human lung fibroblasts»

Abstracts

BOTH NA+/K+-PUMP AND NA+,K+, 2CL- COTRANSPORT CONTRIBUTE TO CELL VOLUME CONTROL IN HUMAN LUNG FIBROBLASTS

Koltsova, S.V.1' 2, Akimova, O.A.2, Orlov, S.N. 1' 2, and Dulin, N.O.3

1 Centre de recherche, Centre hospitalier de l'Université de Montréal (CRCHUM), Montreal, PQ, Canada

2 Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russian Federation

3 Department of Medicine, University of Chicago, Chicago, IL, USA

This study examined the relative impact of Na+/K+-pump and Na+,K+,2Cl- cotransport in the regulation of the volume of human lung fibroblasts. Inhibition of the Na+/K+-pump by ouabain or of Na+,K+,2Cl- cotransport by bumetanide for 24 hours attenuated the rate of K+ (86Rb) influx in human lung fibroblasts from (1537 ± 124) cpm/well in control cells to (695 ± 14) and (522 ± 14) cpm/well in ouabain- or bumetanide-treated cells, respectively, whereas simultaneous addition of both inhibitors decreased the rate of K+ influx to (180 ± 8) cpm/well. Inhibition of either the Na+/K+-pump or of Na+,K+,2Cl- cotransport did not significantly change the volume of human lung fibroblasts, measured as 14C-urea available space, whereas simultaneous blockade of both ion transporters resulted in a 2-fold elevation of cell

volume (table). Thus, our results demonstrate for the first time that both Na+/K+-pump and Na+,K+,2Cl- cotransport contribute to cell volume control in human lung fibro-blasts.

Effect of ouabain and bumetanide on the volume of human lung fibroblasts

+ Cell volume

ml per mg protein pl per cell

None (control) 0.79 ± 0.12 0.23 ± 0.04

Ouabain, 100 nM 0.97 ± 0.14 0.26 ± 0.04

Bumetanide, 10 ^M 0.62 ± 0.01 0.22 ± 0.01

Ouabain + bumet-

anide 1.37 ± 0.13* 0.43 ± 0.04*

* p < 0.05 as compare to controls.

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Бюллетень сибирской медицины, 2013, том 12, № 4, с. 24-68

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