CC BY
29
ISSN 2522-1841 (Online) AZERBAIJAN CHEMICAL JOURNAL № 1 2022 ISSN 0005-2531 (Print)
UDC 544.344; 577.1.08
BIOMIMETIC SENSOR BASED ON Ag-ELECTRODE
N.N.Malikova, N.I.Ali-zade, T.M.Nagiev
M.Nagiev Institute of Catalysis and Inorganic Chemistry, NAS of Azerbaijan
tnagiev@azeurotel.com
Received 07.12.2021 Accepted 24.12.2021
A biomimetic sensor based on an Ag electrode and a smart materia1 TPhPFe3+/Al2O3 has been developed. It has been found that a biomimetic sensor, where (Ag) is used as an electrode, exhibits high sensitivity, stabi1ity and reproducibi1ity. A study was carried out to detect trace concentrations of H2O2 in an aqueous so1ution of TPhPFe3+/A12O3//Ag biomimetic sensor. For (Ag) used as an e1ectrode, the sensitivity threshold was 10-8 wt.%.
Keywords: biomimetic, sensor, tetraphenylporphyrin of iron, catalase, smart material.
doi.org/10.32737/0005-2531-2022-1-29-33
Introduction
One of the promising directions in the field of modern technology is the creation of highly sensitive analytical devices - sensors.
In recent years, sensors have given noticeable advantages in various fields of application: monitoring environmental pollution, assessing the level of industrial pollution in air and water, and also acting a vital role in clinical diagnostics, etc. Therefore, the search for new and effective developments for the preparation of biomimetic sensors is still an urgent problem so far.
Biosensors for enzymatic analysis are characterized by their availability and low cost. Real-time analysis of various analytes is possible with portable and easy-to-use engineering equipment. Due to its high sensitivity and stability, our biomimetic sensor enables to be used for a long time and in any conditions.
The information received from electrochemical sensors, which usually arises from the interaction between the analyte and the electrode elements, is converted into a qualitative or quantitative electrical signal.
Having successful work in the field of imitation catalysis [1, 2], it is possible to prepare biomimetic analogs of the corresponding enzymes, with the help of which many disadvantages of sensors can be eliminated, such as: high cost, high sensitivity to the external environment, multi-stage determination, short operation time, etc.
It was also interesting to consider various materials for the preparation of biosensors.
In [3], ZrO2 is considered. It exists in a variety of structures, including monoclinic, tetragonal, cubic lattice with unique thermal, structural and electronic properties and has excellent natural color, high stability, high viscosity, high chemical strength, desired resistance to corrosion, chemical and microbial attack, which made it a very important advanced ceramic material in terms of its technological aspects.
In the family of nanocarbon graphene oxide, reduced graphene oxide, graphene and carbon nanotubes are prominent new candidates for electrochemical sensors [4].
The use of metal carbides and nitrides for the preparation of biosensors has increased in recent years, and they can potentially replace traditional materials used for gas detection, environmental recovery, photocatalysis, medicine and ceramics, which are very promising for creating electrochemical sensors [5-7].
The search for a metal that can be used in electrochemical sensors to detect various analytes has recently begun to be deposited on alumina, which has become an important material [8].
Previously, we have carried out successful work in this area. For the first time, silver paste, as an adhesive material (to create a contact between the working material and the transducer), was used by us in the study of the phys-icochemical features of the biomimetic sensor,
where Pb was used as a transducer, and TPhPFe3+/Al2O3
was used as a working material [9, 10]. As a result of the studies of the bio-mimetic sensor for catalase activity, it was found that the electrode with TPhPFe3+/AhO3 allows detecting trace concentrations of hydrogen peroxide in an aqueous solution in an
o
amount equal to 10- wt.%.
These successful studies allowed us to continue our prospecting works in the field of catalase-type biomimetic sensors. Semiconductors (Si, Te, Ge), which are widely used in the synthesis of biosensors [11-13], were chosen as the substrates for research.
Materials such as silver, gold and silicon oxide are known to be used as a transducer for the production of biosensors. The use of these materials has led to new methods for the preparation of biosensors. When analysed one of the main advantages of these electrochemical biosensors is the sensitivity and specificity.
In connection with the above, we used Ag as a transducer to prepare a biomimetic sensor. To carry out potentiometric measurements, we used an Ag electrode. TPhPFe , previously adsorbed on Al2O3, was applied to this electrode by gluing (silver paste was used as an adhesive material).
As a result of the research, it has been found that the developed TPhPFe3+/Al2O3//Ag
biomimetic sensor enables to determine the specified concentration within a few seconds.
Experimental part
Experimental studies of the electrode potential of the catalase reaction as a function of time were carried out using the potentiometric method. The electrochemical setup was equipped with a magnetic stirrer to create an equilibrium solution. The background solution is bidistilled water.
Potentiometric studies can be presented in the form of infographics (Figure 1): in a cell filled with a certain amount of bidistilled water (background solution), we determine the e.m.f. of (E) element and then, adding different concentrations of H2O2, the changes in the solution e.m.f. were determined.
The experiments were carried out in a reaction medium consisting of various concentrations of H2O2 aqueous solutions.
Figure 2 shows the experimental data obtained in the study of the catalase activity of
3+
TPhPFe /Al2O3//Ag for biomimetic sensor. From Figure 2, when H2O2 is added to the system, there is a sharp change in the values of the electrochemical potential. Then the value of the electrochemical potential continues to change until the practical decomposition of H2O2. This is evidenced by the fact that the pH value of the solution is 6.8 by the end of the experiment.
Fig. 1. Infographics. AZERBAIJAN CHEMICAL JOURNAL № 1 2022
Fig. 2. Change in e.m.f. systems depending on time at low concentrations of H2O2 for TPhPFe(III)/Al2O3 //Ag biomimetic sensor. 1 - Ag electrode+silver paste, 2 - Ch2o2 =1 wt.%, 3 - Ch2o2 =0.1 wt.%.
AE m -0,14 -0,12 0,1 -0,08 -0,06 -0,04 -0,02 0
t, sec
Fig. 3. Change in e.m.f. systems depending on time at low concentrations of H2O2 for TPhPFe(III)/Al2O3//Ag biomimetic sensor. 1 - CH O =10-4 wt.%,
2 - Ch2o2 =10-6 wt.%, 3 - Ch2o2 =10-8 wt.%.
Fig. 4. Change in e.m.f. systems as a function of time at low concentrations of H2O2 for TPhPFe3+/Al2O3//Ag biomimetic sensor. 1 - Ag electrode + silver paste, 2 - CHzO2 =1 wt.%, 3 - CHzO2 =0.1 wt.%, 4 - CHzO2 =10-4 wt.%, 5 - C^Oz
=10-6 wt.%, 6 - CH O =10-8 wt.%.
This means that the background solution had the same value.
Figure 3 shows the curves versus time at low concentrations of hydrogen peroxide. From Figure 3, a biomimetic sensor prepared by gluing a smart material - TPhPFe3+ onto a transducer -Ag, showed a very high sensitivity. The background solution of H2O for 10-4 wt.% (curve 1), 10-6 wt.% (curve 2) and 10-8 wt.% (curve 3) is (0.042 mV), (0.038 mV) and (0.039), respectively. When H2O2 is added to the system, the values of the electrochemical potential are (0.065 mV) for 10-4 wt.%, (0.065) for 10-6 wt.% and 10-8 wt.%, (0.048) (Figure 3 curves 1 and 3). The sensitivity threshold of TPhPFe3+/Al2O3//Ag of the
o
biomimetic sensor was 10- wt.%.
Figure 4 shows a combined graph of the change in the e.m.f of the system as a function of time at various concentrations of hydrogen peroxide for TPhPFe3+/Al2O3//Ag biomimetic sensor.
Conclusions
This study demonstrates the possibility of using an Ag electrode as a transducer for a bio-mimetic sensor. The study discussed a method for preparing a biomimetic biosensor that showed the lowest detection limit for hydrogen peroxide in aqueous solution.
The developed and synthesized catalase
3+
biomimetic sensor TPhPFe3+/Al2O3//Ag had high sensitivity, stability and the threshold of its sensitivity to trace concentrations of H2O2 was 10-8 wt.%.
The prepared biomimetic sensor is characterized by stability, high sensitivity and reproduc-ibility, with the possibility of expanding the range of determined trace concentrations of H202 in an aqueous solution. The sensor did not lose its activity both under the influence of the oxidizing agent and its intermediates for a long time.
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Ag-ELEKTRODU ÜZORÍNDO BÍOMÍMETÍK SENSOR
N.N.Malikova, N.Leii-zada, T.M.Nagiyev
Ag elektrodu va TPhPFe3+/Al2O3 smart materiali asasinda biomimetik sensor hazirlanmi§dir. Müayyan edilmi§dir ki, (Ag) elektrod kimi istifada olunan biomimetik sensor yüksak hassasliq, sabitlik va takrar istifada qabiliyyatina malikdir. Sulu mahlulda TPhPFe3+/Al2O3//Ag biomimetik sensorun H2O2 iz konsentrasiyalari a§kar etmak ügün tadqiqat apanlmi§dir. Biomimetik sensorun katalaz aktivliyi da 6yranilmi§dir. Ag elektrod transdüser kimi istifada edildikda, hassasliq haddi 10-8 küt.% idi.
Agar sozlar: biomimetik, sensor, damir tetrafenilporfirin, katalaz, smart material.
БИОМИМЕТИЧЕСКИЙ СЕНСОР НА ОСНОВЕ Ag-ЭЛЕКТРОДА
Н.Н.Меликова, Н.И.Али-заде, Т.М.Нагиев
Разработан биомиметический сенсор на основе Ag-электрода и смарт материала - TPhPFe3+/Al2O3. Установлено, что биомиметический сенсор, где в качестве электрода использован (Ag) проявляет высокую чувствительность, стабильность и воспроизводимость. Было проведено исследование обнаружения следовых концентраций H2O2 в водном растворе TPhPFe3/Al2O3//Ag биомиметического сенсора. При использовании в качестве электрода (Ag), порог чувствительности составил 10-8 масс.%.
Ключевые слова: биомиметический, сенсор, тетрафенилпорфирин железа, каталазный, смарт материал.
