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22 • "PROTIST—2016
Optimized U-insertion\deletion search software TAligner and usage of NGS technology enabled us to focus on molecular intermediates of editing process and build detailed editing graphs for all maxicircle cryptogenes. Reconstruction of editing process in cryptogenes showed that in most cases editing can proceed in a variety of alternative ways yielding ORFs with lots of aminoacid substitutions or even with alternative protein product. Our results suggest that editing can be the major driving force ofrapid adaptation to environment changes through the generation of proteins diversity.
GENETIC DIVERSITY OF CENTROHELID HELIOZOA IN BRACKISH INLAND WATERS OF RUSSIA
Gerasimova Elena, Plotnikov Andrey Institute for Cellular and Intracellular Symbiosis UB RAS, Orenburg, Russia [email protected]
Centrohelid heliozoa are ubiquitous, unicellular, non-ciliate phagoheterotrophs with slender radiating axopodia for trapping prey. Centrohelids have a cosmopolitan distribution; inhabit benthos and periphyton in both marine and freshwaters ecosystems, in communities they play role of predators grazing on other microorganisms. At present both genetic and morphological approaches for their taxonomy and classification are used. Morphological and genetic diversity of heliozoa in environmental saline and brackish waters has been studied very poorly. Genetic diversity of centrohelids with group-specific originally designed primers was studied by NGS with MiSeq (Illumina). Fifteen samples from brackish inland water bodies of Russia with mineralization 2-20%c were examined. As a result 40 OTUs were obtained. Most of the environmental OTUs (32) were aligned with Polyplacocystis contractilis (AB196984, NCBI) with support 99% and were placed inside Pterocystina clade. This fact indicates that the most common habitants in brackish waters are related to this particular genetic variant of centrohelida. Other OTUs with 26% and 28% support grouped with environmental centrohelidian sequences from hypersaline habitats and occupied a basal position in Pterocystina A clade.
The research was performed in the Center of Shared Scientific Equipment «Persistence of microorganisms» of ICIS UB RAS and was supported by RSF (14-14-00515) and RFBR (15-2902749,16-44-560234).
BIOGEOGRAPHY AND THE GENETIC STRUCTURE OF MORPHOSPECIES IN THE GENUS MAYORELLA (AMOEBOZOA, DISCOSEA, LONGAMOEBIA) Glotova A.A.
Department ofInvertebrate Zoology, Faculty ofBiology, St. PetersburgState University, Saint Petersburg, Russia [email protected]
Due to the lack of biogeographical data for the majority of naked lobose amoebae genera, the genetic structure of amoebae morphospecies have been previously observed only in several species from Flabellinia group (Amoebozoa, Discosea). Further extensive study of amoebae biogeography including species from different phylogenetic lineages is clearly required given the assumption of amoebae significance for freshwater microhabitat function based on their abundance. In this study Cox I and 18S rDNA genes were used as DNA barcodes in order to identify strains of the genus Mayorella (Discosea, Longamoebia) in addition to morphological data, that allowed to elucidate the morphospecies genetic structure and general principles of their distribution in a global scale and to evaluate resolving power ofvarious identification approaches. The samples were collected from distant freshwater habitats of North America, Europe and Russian Far East. Results contributed to the amoebae morphospecies conception which represents it as a limited set of genetic lineages. Supported with SPSU project 1.38.251.2014
DEEP SEQUENCING OF ANCIENT 18S RDNA
IN SEDIMENT CORES FROM CONTINENTAL
SHELVES OF CHINA
Gong J., Wang Y., Zhu P., Zhang Q.
Yantai Institute of Coastal Zone Research, Chinese
Academy of Sciences, Yantai 264003, China
Protists/phytoplankton in overlying waters and terrestrial biomaterials could be transported, deposited and buried in sediments on continental shelves of marginal seas. Some of these ancient DNA could be preserved, serving as a powerful proxy for reconstructing past regimes of the ecosystems. We obtained three sediment cores each about 2.5 meters in length from three sites at the Bohai Sea (the mouth of Yellow River, shorted as YR) and Yellow Sea (the northern basin, NYS, and southern basins, SYS). The DNA was extracted and quantified and subjected to high throughput sequencing of eukaryotic 18S rDNA fragment using Illumina MiSeq. Classification using pipelines indicated the
