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10THz-I-9
NONLINEAR AND TERAHERTS PHOTONICS
Analysis of the metabolites composition for the ENT organs tissues with high
resolution THz spectroscopy
VVaks1, V.Anfertev1, A.Ayzenshtadt12, M.Chernyaeva1'3, E.Domracheva1, K.Gavrilova1,2
1- Institute for Physics of Microstructures RAS, 603950, Russia, Nizhny Novgorod, GSP-105 2- Children's Municipal Clinical Hospital No.1, 603081, Russia, Nizhny Novgorod, Gagarina av., 76 3- Lobachevsky University, 603022, Russia, Nizhny Novgorod, Gagarina av., 23
Recently, physical and physical-chemical methods of analysis, such as gas chromatography, mass-spectrometry, IR and THz spectroscopy, are becoming increasingly important in medical diagnostics. They allow you to identify substances, the so -called metabolites characteristic of a particular disease. The compilation of a metabolic profile will accelerate and facilitate the process of diagnosis of the disease, evaluate the stage and etiology, as well as predict the therapy. It is extremely relevant to study the state of ear-nose-throat (ENT) organs, since they are one of the main ways of penetrating into the human body of various infections, viruses, fungi, etc. However, there are no data on metabolites in the literature and the Human Metabolome Database in diseases of the ENT organs [1].
The work is devoted to the analysis of the metabolites composition for the ENT organs tissues with high resolution THz spectroscopy and comparing the composition of metabolites that appear during thermal decomposition of a relatively healthy mucosa, a sphenoid sinus polyp and a maxillary sinus cyst. The fast frequency sweeping spectrometer developed by the authors, operating in the range of 118-178 GHz, was used. The frequency range of 60 GHz is covered by the spectrometer in 30 sec. The sensitivity of the recorded absorption coefficient at a cell length of 1 m is from 10-7cm-1 to 5*10-8cm-1. [2, 3]
The identification of substances by absorption lines was carried out using electronic databases [4, 5]. To evaluate the whole metabolites, a qualitative analysis of the chemical composition for thermal decomposition products was performed and a comparison of the resulting spectra of substances and the number of absorption lines of a relatively healthy mucosa, a sphenoid sinus polyp and a maxillary sinus cyst in the frequency range of 60 GHz. If the concentration of the substance in the mixture under study is greater, the more weaker lines will be recorded in the spectrum at the equal conditions (temperature and pressure conditions).
The analysis of the results showed that in all samples there are substances such as methanol, propanediol, acetoni-trile, butyronitrile, benzonitrile, methylmercaptan, azole, ethylene sulfide, carbonyl sulfide and sulfur dioxide. However, in the spectrum of a relatively healthy mucosa, the number of absorption lines of these substances is much less than in the spectra of the polyp and cyst, which indicates their lower concentration. Moreover, in the spectra of polyp and cyst appear absorption lines corresponding such a substances, as acetone, hydroxyaceton, digidroxyaceton, propionitrile, ac-rylonitrile, aminopropionitrile, hydroxyacetonitrile, aminoacetonitrile, methylbutironronitrile, propanal, benzaldehyde, glycolaldehyde, lactaldehyde, malone dialdehyde, monoethanolamine, alanine, pyridine, at that their concentration differ significantly. Besides, thermodecomposition products of cyst contain acetic acid and propenoic acid.
So, we can result, that using THz high resolution spectroscopy for analysis of the metabolites composition for the ENT organs tissues can be a powerful apparatus for metabolic diagnostics.
This research was funded by the Russian Science Foundation, grant No. 21-19-00357, https://rscf.ru/en/proj-ect/21-19-00357/.
[1] D.S. Wishart, Y.D. Feunang, A. Marcu, et al. HMDB 4.0 — The Human Metabolome Database for 2018, Nucleic Acids Res., vol. 46 ( D1), pp. D608-617 (2018).
[2] V.L. Vaks, V.A. Anfertev, V.Yu. Balakirev, et al. High resolution terahertz spectroscopy for analytical applications, Physics Us-pekhi, vol. 63, pp. 708-720, (2020).
[3] V. Vaks, A. Aizenshtadt, V. Anfertev, et al. Analysis of the Thermal Decomposition Products of Pathological and Healthy Tissues in Paranasal Sinuses: A High-Resolution Terahertz Gas Spectroscopy Study. Applied Sciences, vol. 11, pp. 7562 (1-10), (2021).
[4] H.M. Pickett, E.A. Cohen, B.J. Drouin, et al. Submillimeter, Millimeter, and Microwave Spectral Line Catalog. JPL Molecular Spectroscopy, California Institute of Technology, http://spec.jpl.nasa.gov/ftp/pub/catalog/catform.html
[5] C.P. Endres, S. Schlemmer, P. Schilke, et al. The Cologne Database for Molecular Spectroscopy, CDMS, in the Virtual Atomic and Molecular Data Centre, VAMDC, J. Mol. Spectrosc., vol. 327, pp. 95-104, (2016).
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