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1ВОДНЫЕ РАСТВОРЫ В БИОЛОГИЧЕСКИХ СИСТЕМАХ
4
Физика Водных Растворов
Устный доклад
ANALYSIS OF THE KINETICS OF THE GUT MICROBIOTA PRODUCED HYDROGEN AND METHANE BY THE TEST WITH H2- AND CH4-RICH WATER PROBE
Medvedev O.S.,16 Stavrovskii D.B.,2 Bondarenko G.N.,1 Litvinov A.V.,3 Etrekova M.O.,3 Karabinenko A.A.,4 Kilimnik V.A.,5 Ponurovskii Ya.Ya.2
1 Lomonosov Moscow State University, Russia, 119192, Moscow, 27-1 Lomonosovsky Prospekt 2 Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia, 119991, Moscow, 38 Vavilov street
3 National Research Nuclear University "MEPhI", Russia, 115409, Moscow, 31 Kashirskoe shosse
4 Pirogov Russian National Research Medical University, Russia,
117997, Moscow, 1 Ostrovitianov street
5 Saint-Petersburg State University of Aerospace Instrumentation
Russia, 190000 St.Petersburg, 67 Bolshaya Morskaya street 6 Smirnov Institute of Experimental Cardiology Chazov National Medical Research Center for Cardiology, Russia,121552, Moscow, 15A Chazov street e-mail: [email protected]
Introduction. Recent years have seen an upsurge in interest in studying the role of microorganisms of the large intestine (microbiota) of animals and humans and their capacity to generate a number of metabolites that may exert both protective and provocative influence on the development of metabolic, cardiovascular, and other non-infectious diseases. Those food components (primarily complex carbohydrates and dietary fiber) that are not absorbed by our body in the small intestine reach the large intestine, where they are fermented by the microbiota with a much larger set of enzymes for their utilization. As a result of their metabolism, a large number of low-molecular-weight compounds are produced in the colon: gas biomarkers (hydrogen, methane, and hydrogen sulfide), short-chain fatty acids, etc.
In this work, our attention was focused on studying the time dependences of the concentration of hydrogen and methane in the exhalation of the subject after taking two portions of water, which were saturated with hydrogen and methane, respectively. Particular interest in these gases is due to the fact that the analysis of the kinetics of their appearance in the exhaled air allows diagnosing such a disease as bacterial overgrowth syndrome in the small intestine (SIBR). Concentrations of oxygen and carbon dioxide were simultaneously recorded in the exhaled air.
The aim of our study was to obtain experimental data to analyze the process of absorption of H2 and CH4 in the gastrointestinal tract and the process of their release through the lungs.
Methods. Concentrations of CO2 and CH4 were measured by the multichannel diode laser spectrometer, for O2-electrochemical sensor, and for H2-MIS sensor were used [1].
Results. 5 adult volunteers participated in the pilot study. They drank 150 ml portions of water that was saturated with hydrogen and methane at a pressure of 1 atm above the surface of the water. The levels of H2 and CH4 increased in breath by 35-60 ppm with a max on 8-12 min and returned to the baseline level in 45-55 min after drinking H2+CH4-saturated water. The ratio of H2 exhaled/H2 ingested (~60%) is higher than CH4 exhaled/CH4 ingested (~50%), which may reflect different intestinal wall permeability for these gases. Results of the tests with H2- and CH4-saturated water allow to understand better the kinetics of this gaseous biomarkers, generated by the gut microbiota.
Conclusions. First time kinetics of both (hydrogen and methane) release in exhaled air were assessed in humans using multichannel diode laser spectrometer. It is possible to combine this test with taking lactulose or glucose for the regular breath testing because microbiota-produced gases appear in 60-70 min, the time needed to reach the large intestine.
References:
[1] Я.Я. Понуровский, Д.Б. Ставровский, И.В. Широков, Ф.И. Романихин, Г.Н. Бондаренко, А.В. Литвинов, М.О. Этрекова, А.А. Карабиненко, В.А. Килимник, О.С. Медведев. Новые возможности для анализа биологической роли водорода, метана и других биомаркеров активности микробиоты кишечника при использовании перестраиваемой диодной лазерной спек-трометрии поглощения и селективных сенсоров водорода и кислорода// Оптика и спектроскопия, том 132, вып. 3 стр. 303-309 (2024). DOI: 10.61011/QS.2024.03.58150.35-24
