Научная статья на тему 'Alpha glucosidase inhibitory potential of Zataria multiflora extract and its fractions'

Alpha glucosidase inhibitory potential of Zataria multiflora extract and its fractions Текст научной статьи по специальности «Фундаментальная медицина»

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Текст научной работы на тему «Alpha glucosidase inhibitory potential of Zataria multiflora extract and its fractions»

Table 1. Results of antioxidant activity of Rosa canina anthocyanin

Sample DPPH (IC50, ^g/ mL) Nitric oxide scavenging ability (%) (200 ^g/mL) FRAP (IC50, ^g/ mL) ABTS (IC50, ^g/ mL)

Rosa canina Separated by column 476 ± 0.50 53.22 ± 1.25 39 ± 0.03 720 ± 1.2

Rosa canina anthocyanin Separated by column 57 ± 0.57 91.16 ± 0.69 7 ± 0.01 58 ± 0.5

Quercetin 26.51 ± 0.06 - 8.59 ± 0.03 25.64 ± 0.02

5 10

Incubation time (day)

Rosa canina Vit C

Figure 1. Lipid peroxidation of Rosa canina anthocyanin in comparison of Vit C as antioxidant standards

References:

1. Diaconeasa Z, Leopold L, Ruginä D, Ayvaz H, So-caciu C. 2015. Int J Mol Sci. 16(2):2352-265.

2. Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. 2003. Phytochem. 64(5):923-933.

3. Sadeghi H, Hosseinzadeh S, Akbartabar Touri M, Ghavamzadeh M, Jafari Barmak M, Sayahi M, Sa-deghi H. 2016. AJP. 6(2):181-188.

ALPHA GLUCOSIDASE INHIBITORY POTENTIAL OF ZATARIA MULTIFLORA EXTRACT AND ITS FRACTIONS

© Moein M.1,2, Moein S34, Rouzbehan S34, Homaee A.5

1 Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;

2 Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran;

3 Biochemistry Department, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar abbas, Iran;

4 Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar abbas, Iran;

5 Department of Biology, Faculty of Science, University of Hormozgan, Bandar abbas, Iran

Diabetes mellitus is a chronic disease which leads to increase concentrations of glucose in the blood [1]. The WHO projects that diabetes will be the seventh leading cause of death globally in 2030 [2]. One of the therapeutic approaches for diabetes is inhibition of a-glucosidase [3]. Nowadays, some a-glucosidase inhibitors such as, acarbose and voglibose, are widely used but these compounds have side effects [4]. Based on this result, many efforts have been made to search for more effective and natural a-glucosidase inhibitors [5]. In this Study, we have evaluated the a-glucosidase inhibitory potential and inhibition mode of different fractions of Zataria multiflora

Ethanol extract of Zataria multiflora (ZME) was fractionated by liquid-liquid extraction and four fractions

such as (petroleum ether fraction (ZMP), chloroform fraction (ZMC), ethyl acetate fraction (ZMA) and n-butanol fraction (ZMB)) were obtained. a-glucosidase inhibition was determined according to McCue et al method [6]. Acarbose, was used as a positive control. The enzyme kinetics studied by Line weaver burk plot method. Data analysis was performed using one-way analysis of variance (ANOVA).

The results revealed that ZMA was the most inhibitor of a-glucosidase (IC50= 0.35 ± 0.003 mg/ml, Table 1). The curve of Line weaver-Burke plot revealed that all fractions of Zataria multiflora, exhibited noncompetitive-uncompetitive inhibition (Figure 1, Table 2).

Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]

vol. 15/2Q17/suppLeMEnt 1

Table 1. Inhibitory effects of different fractions of Zataria multiflora on a-glucosidase in comparison with acarbose as positive control (IC50 = 11 ± 0.19 mM ~7 mg/ml)

Samples ZMP ZMC ZMA ZMB ZME

IC50 mg/ml 2.5 ± 0.21 2.5 ± 0.21 0.35 ± 0.003 ND ND

ND: no-determined

-ZMB

ZMC -ZMA -ZMP -ZME

Concentration (mg/ml)

X-k-

Figure 1. Inhibition of a-glucosidase by alcoholic extract of Zataria multiflora and its fractions at different concentrations

Table 2. Kinetic parameters of alpha-glucosidase inhibition by crude extract and the fractions of zataria multifura

Condition enzyme assay Vmax (IU)

No inhibitor 1.91

With ZMC 0.81

With ZMP 1.17

With ZMP 0.6

We carried out the first study of Persian edible plant, Zatria multiflora in inhibition of a-glucosidae. Samples included ZMA, ZMC and ZMP (Table 1) were more potent than the positive control acarbose. One advantage of these samples with noncompetitive-uncompetitive inhibitior over acarbose as a competitive inhibitor is that, these samples may not be affected by higher concentrations of the substrate. This study suggests that Zatria multiflora is a promising source of active compounds that can prevent the development of diabetes mellitus type 2.

References:

1. Association AD. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2010; 33 (Supplement 1):S62-S9.

2. Definition W. diagnosis and classification of diabetes mellitus and its complications: report of a WHO consultation. Geneva: WHO. 1999.

3. Gholamhoseinian, Fallah H, Sharifar F, Mirtajaddini M. 2008. Iran J Basic Med Sci. 11 (1):1-9.

4. Shai LJ, Masoko P, Mokgotho MP, Magano SR, Mogale A, Boaduo N et al. 2010. S Afr J Bot. 76(3):465-470.

5. Coman C, Rugina OD, Socaciu C. 2012. Not Bot Hort Agrobot Cluj. 40(1):314-325.

6. Mccue P, KWON YI, Shetty K. 2005. J Food Bio-chem. 29 (3): 278-294.

QUALITY CONTROL AND INVESTIGATION OF MASTIC ADULTERATION IN IRANIAN HERBAL STORE

© Zahra Memariani1, Narjes Gorji1, Reihaneh Moeini1. Sohrab Kazemi2

1 Traditional Medicine and History of Medical Sciences Research Center, Babol University of Medical Sciences, Babol, Iran;

2 Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

Pistacia lentiscus resins, commonly known as mastic, is mostly used as a remedy in Persian traditional medicine. So many mastic samples with unknown origin are sold in Iranian herbal stores.

Purpose of this study is detection possible adulteration in samples found in the markets with a claim of mastic resin aiming towards prevention from counterfeiting.

One mastic sample from Greece (CHIOS MASTIHA GROWERS ASSOCIATION) was considered as a reference. then twelve mastic samples from different cities of Iran and other resins with similar appearance including two synthetic petroleum resins (C5 and C9), colophony resin and Boswellia serrata resin were collected and extracted by n-Hexane. All extracts were

Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1

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