CC BY
13B-I-32
Advances in tissue optical clearing for laser diagnostics and
treatment
Valery V. Tuchin
Saratov State University, Saratov 410012, Russian Federation National Research Tomsk State University, Tomsk 634050, Russian Federation Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov 410028, Russian Federation [email protected]
A description of the optical clearing (OC) technology based on controllable and reversible modification of tissue optical properties by their impregnation with a biocompatible optical clearing agent (OCA) will be done [1-3]. The major mechanisms of OC allowing one to enhance optical imaging and laser treatment facilities of living tissues will be presented. The hyperosmotic properties of OCAs will be discussed in the context of developing a provocative test to investigate the difference between the response of normal and pathological tissue to osmotic stress with the simultaneous optical detection of such a response.
The enhancement of probing/treatment depth and image contrast for a number of human and animal tissues investigated by using different optical modalities, including diffuse reflectance spectroscopy, collimated transmittance, OCT, fluorescence, and Raman microscopies will be discussed [4-8]. Experimental data on the diffusion and permeability coefficients of biocompatible FDA approved OCAs, such as glycerol, e-cigarettes vapes (glycerol/propylene glycol), CT contrast agents (Iohexol and Iodixanol), and MRI contrast agent (Gadobutrol) in skin and mucosal tissues will be presented.
Perspectives of immersion optical clearing/contrasting technique aiming to enhance imaging/treatment of skin and mucosal living tissues by using different imaging and laser treatment modalities will be discussed.
The research was carried out with the support of a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
References
[1] E. A. Genina, et al., Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy, J. Biomed. Photonics & Eng. 1(1), 22-58 (2015).
[2] A. N. Bashkatov et al., Measurement of tissue optical properties in the context of tissue optical clearing, J. Biomed. Opt. 23(9), 091416 (2018).
[3] L. Oliveira, V.V. Tuchin, The optical clearing method: A new tool for Clinical Practice and Biomedical Engineering, Basel: Springer Nature Switzerland AG, 2019.
[4] D.K. Tuchina, I.G. Meerovich, O.A. Sindeeva, V.V. Zherdeva, A.P. Savitsky, A.A. Bogdanov Jr, V.V. Tuchin, Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging. J. Biophotonics 13(11), e201960249 (2020).
[5] Q. Lin, E.N. Lazareva, V.I. Kochubey, Y. Duan, V.V. Tuchin, Kinetics of optical clearing of human skin studied in vivo using portable Raman spectroscopy, Laser Physics Letters 17 (10), 105601(2020).
[6] D.K. Tuchina, I.G. Meerovich, O.A. Sindeeva, V.V. Zherdeva, N.I. Kazachkina, I.D. Solov'ev, A.P. Savitsky, A.A. Bogdanov Jr, V.V. Tuchin, Prospects for multimodal visualization of biological tissues using fluorescence imaging, Quantum Electronics 51(2), 104-117 (2021).
[7] N.I. Kazachkina, V.V. Zherdeva, A.N. Saydasheva, I.G. Meerovich, V.V. Tuchin, Savitsky A.P., Bogdanov, A.A. Topical gadobutrol application causes fluorescence intensity change in RFP-expressing tumor-bearing mice, Journal of Biomedical Photonics & Engineering 7 020301(2021).
[8] I. Carneiro, S. Carvalho, R. Henrique, A. Selifonov, L. Oliveira, V.V. Tuchin, Enhanced ultraviolet spectroscopy by optical clearing for biomedical applications, IEEE Journal of Selected Topics in Quantum Electronics 27 (4), 1-8 (2021).
