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12Results and discussion Lcssy effective component have significant effective in inhibit ovarian cancer tumor growth, the dose group is the best compared with the model group(P<0.01);The analysis of gene mi-croarray show that there are four hundred differentiated lncRNAs.Co-expressed mRNAs for differentiated lncRNAs are enriched in the functional terms of JAK/STAT Signaling pathway and STAT3 transcription factor.Since STAT3 signaling pathway plays an important role in tumor angiogenesis,it is speculated that LCSSY may regulate the expression of STAT signaling pathway by regulating the expression of lncRNA in ovarian cancer.
Key words: LCSSY,effective component,ovarian cancer,LncRNA,gene chip
Reference:
[1]ChengKun,HanFengjuan,WuXiaoke. The effect of the method of removing kidney essence and removing essence on reducing the toxicity of chemotherapy in patients with ovarian cancer after chemotherapy [J].TianJin Journal Of Traditional Chinese Medi-cine.2010,27(3):215-216.
[2]QiuLinan,HanFengjuan,WuXiaoke,HouLihui,ChenHaili. Inhibitory effect of Chong Xue Yin Xue Yin on Fischer344 rat ovarian tumor and its effect on vEgF expression [J].World Journal of integrated Traditional and Western Medicine.2010,5(11):939-941.
[3]HanFengjuan,XuFeng. Study on the effect of Chong Chong Yu Yin on the expression of P53 and PCNA in rat ovarian tumor [J]. Chinese Journal of Tradional Medicine Science and Techsology.2007,14(6):402.
[4]ZhangYan. Study on the effect of the effective components of LCSSY on the angiogenesis of ovarian cancer and the regulation mechanism mechanism of JAK2/STAT3 signaling pathway.
[D]. Heilongjiang University Of Chinese Medicine,2016.
[5] Guttman, M., et al., Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature, 2009. 458(7235): p. 223-7.
A CLINICAL STUDY OF ACUPUNCTURE COMBINED WITH REHABILITATION TRAINING IN THE TREATEMT OF SHOULDER-HAND SYNDROME AFTER STROKE
Zhao bin
The Second Hospital Affiliated Heilongjiang University of Chinese Medicine
Abstract Objective To observe the effects of acupuncture combined with rehabilitation training on neurological function ,upper limb motor function,pain degree, comprehensive function in the patients with shoulder-hand syndrome(SHS) after stroke. To study the therapeutic effects of acupuncture combined with rehabilitation training on SHS after stroke and to supply the Evidence of its clinical application.
Methods All the cases were the inpatients of the second hospital affiliated heilongjiang university of chinese medicine. 90 cases of shoulder-had syndrome after stoke enrolled according to inclusion criteia were randomized into three groups : the acupuncture group(groupA),the rehabilitation training group (groupB) and the acupuncture combined with rehabilitation training group (groupC) ,30 cases in each group . Patients in the acupuncture group (groupA) were treadted only with acupuncture , patients in the rehabilitation training group (groupB) were tread-ted o nly with rehabilitation training, and the patients in acupuncture combined with rehabilitation training group (groupC) were treated with both acupuncture and rehabilitation training . The treatment course lasted for 28 days, acupuncture and rehabilitation training were given five days a week , and basic drug therapy was given throughout the whole course in all the three groups . The three group were evaluated before teratment , 14 days after the treatment and after the whole treatment course .Neurological function was evaluated by Neural Dysfunction Scale (NDS) , upper limb motor function was evaluated by Simplified Fugl-Meyer Assessment (FMA),pain degree was evaluated by Visual Anlogue Scale (VAS) , and comprehensive function was evaluated by Funtion-al Comprehensive Assessment (FCA) . The comprehensive effect was evaluated after the treatment cuurse.
Resulte Before the treatment , there was no significant difference of age ,sex , NDS score, Simpified FMA score , VAS score and FCA score between three groups(P>0.05)
1. Results of NDS : Compared with before treatment, the NDS scores decreased significantly both after 14 days of treatment and after the whole treatment course in all the three groups (P<0.01) . By the reduced scores of NDS af-ther 14 days of treatment and after the whole treatment course from higher to lower, three groups were sorted as : the acupuncture combined with rehabilitation training group (groupC) , the acupuncture group (groupA) and the rehabilitation training group ( groupB ) . There was no significant difference between group C and group A (P>0.05) . The difference was significant between group C and group B , as well as between group A and group B (P<0.01) .
2 . Results of Simplified FMA : Compared with before treatment , the Simplified FMA score incrsased significantly both after 14 days of treatment and after the whole treatment course in all three groups (P<0.01) . By the incrsased score of Simplified FMA after 14 days of treatment and after the whole treatment course from higher to lower, three groups were sorted as :groupC,group A and group B.There was no significant difference between three :groups after 14 days of treatment ( P>0.05 ) . The incrsased score of group C was significantly higher than group B ( P<0.01) ,and the incrsased score of group A was also significantly higher than group B( P>0.05 ). There was no significant Difference between group C and group A ( P>0.05 ). 106 Амурский медицинский журнал №3 (19) 2017
3 . Results of VAS : Compared with before treatment , the VAS score decreased significantly after 14 days of treatment and after the whole treatment course in all the three groups ( P<0.01) . By the reduced scores of VAS after 14 days of treatment and after the whole treatment course from higher to lower . The three groups were sorted as : group C , group A and group B . There was no significant difference between group C and group A( P>0.05 ) . The difference was significant between group C and group B ( P<0.01) , as well as between group A and group B ( P<0.05 ).
4 . Results of FCA : Compared with before treatment , the FCA score increased significantly both after 14 days of treatment and after the whole treatment course in all the three groups ( P<0.01) . By the increaed scores of FCA after 14days of treatment and after the whole treatment course from higher to lower, the three groups were sorted as : group C , group A and group B . There was no significant difference between group C and group A( P>0.05 ) . the difference was significant between group C and group B , as well as between group A and group B ( P<0.01).
5 . Results of the comprehensive effect evaluatiaon : Apparent rate of the three groups was : group A 26.7%, group B 3.3% and group C 36.7%.Total effective rate of the three groups was : group A90.0%, group B 83.3% and group C 96.7%. By the effect of therapy from better to worse three groups were sorted as:group C ,group A and group B. There was no significant difference between groupC and group A( P>0.05 ),and the difference was significant between group C and group B ( P<0.01),as well as between group A and group B ( P<0.05 ).
Conclusion Acupuncture , rehabilition training and acpuncture combined with rehabilition training can all improve the neurological function, upper limb motor function and comprehensive function and release pain pf patients with shoulder-hand syndrome after stroke.Acupuncture combined with rehabilition training is significantly more effective than simple rehabilition training.Although the difference was not significant,acpuncture combined with rehabilition training improved the condition better than simple acpuncture,and the difference was more obvious as the treatment course extended.There is a synergetic effect of acupuncture and rehabilition training in the therapy of SHS after stroke.
Key words storke: shoulder-hand syndrome; accpuncture;rehabilition training
PREPARATION OF SOLID SELF-EMULSIFYING SYSTEM FOR POORLY WATER-SOLUBLE DRUG SOPHORAFLAVANONE G
Zhixin Yang*, Jiajia Liu, HaiweiWang, Zhang Lei, Wang Xin
(College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China)
AbstractSFG hasso many pharmacological activitiesthat SFG may become a promising new molecular entity (NME), but its waterinsolubleaffectsitsapplication.Bythisexperiment,SFGwasmadeintoanewsolidself-emulsifyingsystem(S-SMEDDS) toincreasethedissolution andabsorptionof SFGandevaluatethebioavailabilityin Sprague-Dawley(SD)ratsbyoralgavage. Thispaper provides amethod to improve its solubility inorderto provide areference for theresearch andapplicationofSFG.
Key words: Sophoraflavanone G; self-emulsifying system bioavailability
Introduction Sophoraflavanone G(SFG) is for leguminous plants ofSophora flavescens flavonoids.Studies have shown that flavescens flavonoids havea good antibacterial activity[1], anti-inflammatory[2], anti-malarial and the role of en-zymes[3]. However, the hydrophobicity of the isopentenyl compound may affect its bioavailability in vivo to a certain extent.Self-emulsifying drug delivery system (SMEDDS) is a solid or liquid formulation that contains an oil phase, an emulsifier, and a co-emulsifier, and is a lipid delivery system [4].Therefore ,in this experiment, SFG was made into solid self-emulsifying system to increase both the dissolution and the absorption ,while improving its bioavailability.
Materials and methods SFG was synthetic by the Heilongjiang University of Traditional Chi-
nese Medicine (China). Methanol (chromatographic purity, Fisher Co., Ltd., USA)All other chemicals and solvents used were of analytical reagent grade.Healthy male SD rats, weighing 240-260g,w-ere provided by Experimental Animal Center of Heilongjiang University of Chinese Medicine.
UPLC analysis of SFG The UPLC method was used for the determination of SFG in the solubility test, dissolution test, and pharmacokinetic studyin vivo/vitro. The mobile phase was methanol /water at the ratio of 4:1. The wavelength of the UV detector, flow rate, and injection volume were set at 294 nm, 0.2mL/min and 3^L, respectively.
Formulation and preparation of Sophoraflavanone G S-SMEDDS
Several formulations of SMEDDS were prepared containing a fixed proportion of SFG (20.0 mg/g) dissolved in a mixture of vehicles composed of cremophor RH40(emulsifier), PEG400 (co-emulsifier) and Ethyl Oleate as oil phase .These components were accurately weighed and mixed using a magnetic stirrer until a clear solution was obtained. All of the liquid formulations were stored in air-tight glass containers at -4°C until required for use as below .
In vitro releasestudy and In vivo pharmacokinetic study
The optimum formulation SFG and SFG-S-SMEDDS were weighed, and 900 mL of distilled water was used as the dissolution medium at a temperature of (37±0.5)°C, and the speed was50rpm/min, respectively, at 5,10,20,30,45,60,120 min when the sample solution,determination of SFG content, calculate the cumulative dissolution.
Twelve SD rats were divided into two groups,which wereorally administrated optimized SFG S-SMEDDS
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