Биотехнологии в медицине список научных статей
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Gene Therapy for Cystic Fibrosis: Recent Advances and Future Prospects
Gene replacement therapies are novel therapeutic approaches that seek to tackle hereditary diseases caused by a congenital deficiency in a particular gene, when a functional copy of a gene can be delivered to the cells and tissues using various delivery systems. To do this, viral particles carrying ...
2023 / М А. Lomunova, P. M. GershovichWOSScopusВАКPubMed
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MALAT1 Long Non-coding RNA and Its Role in Breast Carcinogenesis
Our genome consists not only of protein-coding DNA, but also of the non-coding part that plays a very important role in the regulation of all cellular processes. A part of the non-coding genome comes with non-coding RNAs (ncRNAs), and disruption of the functional activity of these RNAs may be...
2023 / M. M. Tsyganov, M. K. IbragimovaWOSScopusВАКPubMed -
Relationship between the Gene Expression of Adenosine Kinase Isoforms and the Expression of CD39 and CD73 Ectonucleotidases in Colorectal Cancer
Tumor cells have the capacity to create an adenosine-rich immunosuppressive environment, which can interfere with antitumor immunotherapy. Approaches are currently being developed with a view to suppressing the production of adenosine or its signals. Such approaches include the use of antibodies to ...
2023 / G. A. Zhulai, M. I. ShibaevWOSScopusВАКPubMed -
literammp9 mRNA Expression and Bridging Fibrosis Progression in Toxic Liver Injury
Developing liver disease treatments, in which fibrosis is a key pathogenetic link, still remains an urgent problem in hepatology. In the present study, the level of mmp-9 mRNA expression and the number of FAP+, α-SMA+, CD45+ cells were analyzed at nine time points of fibrosis and cirrhosis. It was...
2023 / E. I. Lebedeva, A. S. Babenka, A. T. ShchastniyWOSScopusВАКPubMed -
EGFR Suppression Inhibits the Sphere Formation of MCF7 Cells Overexpressing EGFR
The epidermal growth factor receptor (EGFR) is an oncogenic tyrosine kinase that is involved in tumor initiation and progression, making EGFR inhibitors and monoclonal antibodies to this receptor essential for anti-tumor therapy. We have previously shown that EGFR transgene expression in the human...
2023 / D. D. Novak, O. S. Troitskaya, A. A. Nushtaeva, M. V. Zhilnikova, V. A. Richter, M. I. Meschaninova, O. A. KovalWOSScopusВАКPubMed -
CRISPR/Cas9 Essential Gene Editing in Drosophila
Since the addition of the CRISPR/Cas9 technology to the genetic engineering toolbox, the problems of low efficiency and off-target effects hamper its widespread use in all fields of life sciences. Furthermore, essential gene knockout usually results in failure and it is often not obvious whether...
2023 / I. S. Osadchiy, S. O. Kamalyan, K. Y. Tumashova, P. G. Georgiev, O. G. MaksimenkoWOSScopusВАКPubMed -
Cell Therapy: A New Technology for Cerebral Circulation Restoration after Ischemia/Reperfusion
Cell therapy with mesenchymal stem cells (MSCs) may be a promising technique for cerebral blood flow restoration after transient ischemia. Before a practical application of the cell material, 7–9 days are required for its cultivation. We studied the efficacy of human MSC (hMSC) transplantation...
2023 / I. B. Sokolova, O. P. GorshkovaWOSScopusВАКPubMed -
Генная терапия муковисцидоза: достижения и перспективы
Одним из современных подходов к терапии наследственных заболеваний, вызванных дефицитом того или иного гена в организме, является заместительная генная терапия, которая заключается в том, что функциональную копию гена вводят в клетки и ткани с помощью различных систем доставки. С этой целью могут...
2023 / М А. Ломунова, П М. ГершовичWOSScopusВАКRSCIPubMed -
Клеточная терапия – новая технология восстановления церебральной циркуляции после ишемии/реперфузии
Клеточная терапия с использованием мезенхимных стволовых клеток (МСК) может быть перспективным методом восстановления мозгового кровотока после транзиторной ишемии. Клеточный материал для его практического применения необходимо культивировать в течение 7–9 сут. В работе изучена эффективность...
2023 / И Б. Соколова, О П. ГоршковаWOSScopusВАКRSCIPubMed -
Attenuating Neuronal Autophagy Alleviates Inflammatory Injury in OGDDeprived Co-culture of HT22 with BV2
Neuronal CX3CL1 suppressed microglial inflammation by binding to its receptor CX3CR1 expressed on microglia. Neuronal autophagy was prominently activated by cerebral ischemia, whereas CX3CL1 expression in autophagic neurons was conversely down-regulated to exacerbate microglial inflammation....
2023 / Z. W. Huang, Y. Y. Liu, X. M. Chen, C. L. Yu, H. Y. He, Y. H. DengWOSScopusВАКPubMed -
Soft X-ray Microscopy in Cell Biology: Current Status, Contributions and Prospects
The recent advances achieved in microscopy technology have led to a significant breakthrough in biological research. Super-resolution fluorescent microscopy now allows us to visualize subcellular structures down to the pin-pointing of the single molecules in them, while modern electron microscopy...
2023 / S. A. Golyshev, E. P. Kazakov, I. I. Kireev, D. G. Reunov, I. V. MalyshevWOSScopusВАКPubMed -
The Potential and Application of iPSCs in Gene and Cell Therapy for Retinopathies and Optic Neuropathies
This review focuses on in vitro modeling of diseases and the development of therapeutic strategies using iPSCs for the two most common types of optical pathologies: hereditary neuropathies and retinopathies. Degeneration of retinal ganglion cells and the subsequent optic nerve atrophy leads to...
2023 / Е V. Lapshin, Y. G. Gershovich, A. V. KarabelskyWOSScopusВАКPubMed -
RIPK3 Expression in Fibroblasts in an in vivo and in vitro Skin Wound Model: A Controversial Result
One of the major problems of regenerative medicine is the development of hypertrophic scars and keloids. The protein kinase RIPK3 is involved in necroptosis; however, recent evidence indicates that it also has non-canonical functions, including its involvement in the development of renal fibrosis....
2023 / I. S. Izumov, M. S. Shitova, M. S. Sabirov, S. A. Sheleg, O. L. Cherkashina, E. P. Kalabusheva, E. A. Vorotelyak, E. I. MorgunWOSScopusВАКPubMed -
BODIPY Dye Derivative for Irreversible Fluorescent Labeling of Eukaryotic Cells and Their Simultaneous Cytometric Analysis
In this work, we synthesized a green fluorescent dye derivative, 1,3,5,7-tetramethyl-BODIPY, with a heptyl substituent at the 8-position. The obtained highly hydrophobic compound was able to rapidly and irreversibly bind to eukaryotic cells. Incubation of cells with the dye over different periods...
2023 / A. Yu. Frolova, S. V. Kutyakov, V. I. Martynov, S. M. Deyev, A. A. PakhomovWOSScopusВАКPubMed -
System for Self-excited Targeted Photodynamic Therapy Based on the Multimodal Protein DARP-NanoLuc-SOPP3
Despite the significant potential of photodynamic therapy (PDT) as a minimally invasive treatment modality, the use of this method in oncology has remained limited due to two serious problems: 1) limited penetration of the excitation light in tissues, which makes it impossible to affect deep-seated ...
2023 / E. I. Shramova, A. Yu. Frolova, V. P. Filimonova, S. M. Deyev, G. M. ProshkinaWOSScopusВАКPubMed -
Membrane Partitioning of TEMPO Discriminates Human Lung Cancer from Neighboring Normal Cells
The plasma membranes of normal and cancer cells of the lung, breast, and colon tissues show considerably different lipid compositions that greatly influence their physicochemical properties. Partitioning of the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) into the membranes of human lung ...
2023 / O. K. Gasymov, M. J. Bakhishova, R. B. Aslanov, L. A. Melikova, J. A. AliyevWOSScopusВАКPubMed -
Хромосомные аберрации в эмбриональном развитии человека как биологическое явление
Отличительной чертой раннего эмбрионального развития млекопитающих, особенно человека, являются частые хромосомные аномалии. Анеуплоидии считаются фактором, способствующим неудачной имплантации эмбриона и спонтанным абортам. Влияние хромосомного мозаицизма на способность эмбрионов к нормальному...
2023 / А Д. Иванова, М Л. СемёноваWOSScopusВАКRSCIPubMed -
Attenuating Neuronal Autophagy Alleviates Inflammatory Injury in OGDDeprived Co-culture of HT22 with BV2
Neuronal CX3CL1 suppressed microglial inflammation by binding to its receptor CX3CR1 expressed on microglia. Neuronal autophagy was prominently activated by cerebral ischemia, whereas CX3CL1 expression in autophagic neurons was conversely down-regulated to exacerbate microglial inflammation....
2023 / Z. W. Huang, Y. Y. Liu, X. M. Chen, C. L. Yu, H. Y. He, Y. H. DengWOSScopusВАКRSCIPubMed -
Потенциал и использование индуцированных плюрипотентных стволовых клеток в генной и клеточной терапии ретинопатий и оптических нейропатий
В представленном обзоре на примере двух наиболее распространенных типов патологии зрительной системы – наследственной оптической нейропатии и ретинопатии – рассмотрены in vitro моделирование этих заболеваний с использованием индуцированных плюрипотентных стволовых клеток (ИПСК), а также подходы к...
2023 / Е В. Лапшин, Ю Г. Гершович, А В. КарабельскийWOSScopusВАКRSCIPubMed -
Membrane Partitioning of TEMPO Discriminates Human Lung Cancer from Neighboring Normal Cells
The plasma membranes of normal and cancer cells of the lung, breast, and colon tissues show considerably different lipid compositions that greatly influence their physicochemical properties. Partitioning of the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) into the membranes of human lung ...
2023 / O. K. Gasymov, M. J. Bakhishova, R. B. Aslanov, L. A. Melikova, J. A. AliyevWOSScopusВАКRSCIPubMed
