К ВОПРОСУ О ПРИМЕНЕНИИ ХЛОРОФИЛЛИНА В КАЧЕСТВЕ РАДИОЗАЩИТНОГО СРЕДСТВА

Ромодин Леонид Александрович; Московский Александр Александрович; Митрофанова Антонина Васильевна

�� . 2024. � 2. �. 49 � 58

�he Veterinarian. 2024; (2): 49 � 58

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�� 615.07

DOI: 10.33632/1998-698�_2024_2_49

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�� 1, �� , rla2904@mail.ru

�� 2, moskowsky.sch858@gmail.com

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�� : �� .�., �� .�., �� .�.. � �� // �� . 2024 . �2 . �. 49-58 . DOI: 10.33632/1998-698�_2024_2_49

ON THE ISSUE OF THE USE OF CHLOROPHYLLIN AS A RADIOPROTECTIVE AGENT

Leonid A. Romodin1, candidate of biological sciences, rla2904@mail.ru

Aleksandr A. Moskovskij2, moskowsky.sch858@gmail.com

Antonina V. Mitrofanova1,89123454833@mail.ru

1State Research Center � Burnasyan Federal Medical Biophysical Center of Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russian Federation

2Federal State Budgetary Educational Institution of Higher Education Russian Biotechnological University, Moscow, Russian Federation

Corresponding author: Leonid Aleksandrovich Romodin.

Abstract. Despite the fact that the active search and development of radioprotective drugs have been conducted since the middle of the last century, this problem cannot be considered solved. This is due to the fact that all currently recognized effective radioprotectors are chemically toxic. Therefore, a search is being conducted for potential radioprotective drugs among non-toxic or low-toxic natural compounds. There is extensive information in the literature regarding these substances. But most of them are scattered and unsystematic. Most of these works do not provide any data or even assumptions about the mechanism of the radioprotective effect of the drugs presented. And the lack of information about the mechanisms of action of the substance and its metabolic pathways complicate further research to find the most clinically acceptable and effective form of the drug. There is information in the literature that chlorophyll-based drugs may have radioprotective properties. There are especially many reports on the water�soluble product of its saponification - chlorophyllin. The most commonly used copper complex of chlorophyllin is much more stable compared to the magnesium or nonmetallic form. This work is devoted to the study of the properties of chlorophyllin, the concepts of which are necessary for the development of a clinically acceptable form of the drug. In the course of the work, the optical properties of copper chlorophyllin were studied: the molar extinction coefficient at the absorption maximum (? = 405 ��) is 33800 l/mol�cm. The data obtained are necessary for the correct assessment of the dosage of the drug. We have explored a molecular model by using the chemiluminescence registration method. It was found that the suppression of lipid peroxidation reactions can play an important role in the mechanism of the radioprotective effect of chlorophyll-based drugs. We used a suspension of lymphocytes by spectrophotometry. It was shown that copper chlorophyllin is able to penetrate into cells. This greatly facilitates the development of ways to deliver the drug to target cells.

Keywords: ionizing radiation, radioprotector, chlorophyll, chlorophyllin, lipid peroxidation, spectrophotometry

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1. Singh, V. K. The efficacy and safety of amifostine for the acute radiation syndrome / V. K. Singh, T. M. Seed // Expert opinion on drug safety. � 2019. � Vol. 18, � 11. � P. 1077�1090. doi: 10.1080/14740338.2019.1666104.

2. ��, �. �. �� -190 (��) � �� / �. �. �� // �� . ��. � 2020. � �. 60, � 4. � �. 378�395. doi: 10.31857/S0869803120040128.

3. ��, �. �. �� / �. �. �� // �� . ��. � 2013. � �. 53, � 5. � �. 459�467. doi: 10.7868/S0869803113050160.

4. Paladino, M. E., Iodine thyroid blocking. A lesson from the worst nuclear accidents in history / M. E. Paladino, M. Belingheri, R. Mazzagatti, M. A. Riva // Internal and emergency medicine. � 2022. � Vol. 17, � 8. � P. 2449�2450. doi: 10.1007/s11739-022-03065-y.

5. ��, �. �. �� / �. �. ��, �. �. ��, �. �. ��, �. �. ��, �. �. ��, �. �. �� // �� . � 2020. � �. 65, � 3. � �. 66�72. doi: 10.12737/1024-6177-2020-65-3-66-72.

6. ��, �. �. �� / �. �. ��, �. �. �� // �� . ��. � 2019. � �. 59, � 2. � �. 132�149. doi: 10.1134/S0869803119020085.

7. ��, �. �. �� : �� / �.�. �� // �� . ��. � 2020. � �. 60, � 3. � �. 279�290. doi: 10.31857/S086980312003011X.

8. ��, �. �. �� / �. �. ��, �. �. �� // �� . � 2018. � �. 2, � 42. � �. 60�62.

9. ��, �. �. �� / �. �. ��, �. �. �� // �� . � 2012. � �2. � �. 107�109.

10. ��, �. �. �� / �. �. �� // �� . � 2013. � �7. � �. 53�54.

11. Zimmering, S. Evidence for a radioprotective effect of chlorophyllin in Drosophila / S. Zimmering, O. Olvera, M. E. Hernandez, M. P. Cruces, C. Arceo, E. Pimental // Mutation research. � 1990. � Vol. 245, � 1. � P. 47�49. doi: 10.1016/0165-7992(90)90024-e.

12. Abraham, S. K. Role of chlorophyllin as an in vivo anticlastogen: protection against gamma-radiation and chemical clastogens / S. K. Abraham, L. Sarma, P. C. Kesavan // Mutation research. � 1994. � Vol. 322, � 3. � P. 209�212. doi: 10.1016/0165-1218(94)90008-6.

13. Morales-Ramirez, P. In vivo effect of chlorophyllin on gamma-ray-induced sister chromatid exchange in murine bone marrow cells / P. Morales-Ramirez, M. C. Garcia-Rodriguez // Mutation research. � 1994. � Vol. 320, � 4. � P. 329�334. doi: 10.1016/0165-1218(94)90085-x.

14. Morales-Ramirez, P. In vivo radioprotective effect of chlorophyllin on sister chromatid exchange induction in murine spermatogonial cells // P. Morales-Ramirez, M. T. Mendiola-Cruz // Mutation research. � 1995. � Vol. 344, � 1-2. � P. 73�78. doi: 10.1016/0165-1218(95)90041-1.

15. Kumar, S. S. Effect of chlorophyllin against oxidative stress in splenic lymphocytes in vitro and in vivo / S. S. Kumar, B. Shankar, K. B. Sainis // Biochimica et biophysica acta. � 2004. � Vol. 1672, � 2. � P. 100�111. doi: 10.1016/j.bbagen.2004.03.002.

16. Ozcan, M. Protective Effects of Antioxidant Chlorophyllin in Chemically Induced Breast Cancer Model In vivo / M. Ozcan, D. Aydemir, M. Bacanli, H. G. Anlar, N. N. Ulusu, Y. Aksoy // Biological trace element research. � 2021. � Vol. 199, � 12. � P. 4475�4488. doi: 10.1007/s12011-021-02585-6.

17. Gomes, B. B. Bioavailability of dietary sodium copper chlorophyllin and its effect on antioxidant defence parameters of Wistar rats / B. B. Gomes, S. B. Barros, E. R. Andrade-Wartha, A. M. Silva, V. V. Silvab, U. M. Lanfer-Marquez // Journal of the science of food and agriculture. � 2009. � Vol. 89. � P. 2003�2009. doi: DOI 10.1002/jsfa.3681.

18. Hayes, M. Update on the bioavailability and chemopreventative mechanisms of dietary chlorophyll derivatives / M. Hayes, M. G. Ferruzzi // Nutrition research. � 2020. � Vol. 81. � P. 19�37. doi: 10.1016/j.nutres.2020.06.010.

19. Sciuti, L. F. Picosecond dynamic of aqueous sodium-copper chlorophyllin solution: An excited state absorption study / L. F. Sciuti, L. H. Z. Cocca, A. R. L. Caires, P. J. Goncalves, L. de Boni // Chemical Physics Letters. � 2018. � Vol. 706. � P. 652�657. doi: https://doi.org/10.1016/j.cplett.2018.07.016.

20. ��, �. �. ��-�� / �. �. �� // ��: ��, � 1986. � 282 �.

21. ��, �. �. ��, �� , � �� ; ��, �� / �. �. ��, �. �. ��, �. �. �� // �� . � 2014. � �. 6, � 4. � �. 4�25.

22. ��, �. �. �� . �� 1 / �. �. �� // ActaNaturae. � 2021. � �. 13, � 3. � �. 90�100. doi: 10.32607/actanaturae.10912.

23. Sharov, V. S. Assessment of the C-525 laser dye as a chemiluminescence sensitizer for lipid peroxidation in biological membranes: a comparison with chlorophyll-a / V. S. Sharov, K. Briviba, H. Sies // Free radical biology & medicine. � 1996. � Vol. 21, � 6. � P. 833�843.

24. Vlasova, I. I. Peroxidase Activity of Human Hemoproteins: Keeping the Fire under Control / I. I. Vlasova // Molecules. � 2018. � Vol. 23, � 10. � P. 2561. doi: https://doi.org/10.3390/molecules23102561.

25. Yao, Y. The effect of radio frequency heating on the inactivation and structure of horseradish peroxidase / Y. Yao, B. Zhang, H. Pang, Y. Wang, H. Fu, X. Chen // Food chemistry. � 2023. � Vol. 398. � P. 133875. doi: 10.1016/j.foodchem.2022.133875.

26. Sun, F. HRG-9 homologues regulate haem trafficking from haem-enriched compartments / F. Sun, Z. Zhao, M. M. Willoughby, S. Shen, Y. Zhou, Y. Shao, J. Kang, Y. Chen, M. Chen, X. Yuan, I. Hamza, A. R. Reddi, C. Chen // Nature. � 2022. � Vol. 610, � 7933. � P. 768�774. doi: 10.1038/s41586-022-05347-z

References

1. Singh, V. K. The efficacy and safety of amifostine for the acute radiation syndrome / V. K. Singh, T. M. Seed // Expert opinion on drug safety. � 2019. � Vol. 18, � 11. � P. 1077�1090. doi: 10.1080/14740338.2019.1666104.

2. Vasin, M. V. B-190 (indralin) in the light of history of formation of ideasof the mechanism of action of radioprotectors / M. V. Vasin // Radiacionnaya biologiya. Radioekologiya. � 2020. � Vol. 60, � 4. � P. 378�395. doi: 10.31857/S0869803120040128.

3. Vasin, M. V. The Classification of Radiation Protective Agents as the Reflection of the Present State and Development Perspective of Current Radiation Pharmacology / M. V. Vasin // Radiacionnaya biologiya. Radioekologiya. � 2013. � Vol. 53, � 5. � P. 459�467. doi: 10.7868/S0869803113050160.

4. Paladino, M. E., Iodine thyroid blocking. A lesson from the worst nuclear accidents in history / M. E. Paladino, M. Belingheri, R. Mazzagatti, M. A. Riva // Internal and emergency medicine. � 2022. � Vol. 17, � 8. � P. 2449�2450. doi: 10.1007/s11739-022-03065-y.

5. Metlayev, E. G. International and national approaches to the conducting of iodine thyroid blocking in the accident of a nuclear reactor / E. G. Metlayev, L. S. Bogdanova, M. I. Grachev, Yu. A. Salenko, G. P. Frolov, A. M. Lyagnynskaya // Medical radiology and radiation safety. � 2020. � Vol. 65, � 3. � P. 66�72. doi: 10.12737/1024-6177-2020-65-3-66-72.

6. Grebenyuk, A. N. Modern Condition and Prospects for Development of Medicines for Prevention and Early Treatment of Radiation Injures / A. N. Grebenyuk, V. D. Gladkikh // Radiation biology. Radioecology. � 2019. � Vol. 59, � 2. � P. 132�149. doi: 10.1134/S0869803119020085.

7. Rozhdestvensky, L. M., Difficulties in Radiation Counter Measure Preparations Development in Russia in Crysis Period: Actual Approaches Searching / L. M. Rozhdestvensky // Radiation biology. Radioecology. � 2020. � Vol. 60, � 3. � P. 279�290. doi: 10.31857/S086980312003011X.

8. Pozdeev, A. V. Increase in radiation stability of an organism mammals at use of medicines of a chlorophyll in the conductions of radioactive environmental pollution / A. V. Pozdeev, N. P. Lysenko // Izvestiya Mezhdunarodnoj akademii agrarnogo obrazovaniya � 2018. � Vol. 2, � 42. � P. 60�62.

9. Pozdeev, A. V. The effect of the chlorophyll preparation on the content of malondialdehyde in radiation pathology / A. V. Pozdeev, V. P. Gugalo // Vestnik Kurskoj gosudarstvennoj sel'skohozyajstvennoj akademii. � 2012. � �2. � P. 107�109.

10. Pozdeev, A. V. Experimental study of the cortisol content in the blood during radiation exposure / A. V. Pozdeev // Vestnik Kurskoj gosudarstvennoj sel'skohozyajstvennoj akademii. � 2013. � �7. � P. 53�54.

11. Zimmering, S. Evidence for a radioprotective effect of chlorophyllin in Drosophila / S. Zimmering, O. Olvera, M. E. Hernandez, M. P. Cruces, C. Arceo, E. Pimental // Mutation research. � 1990. � Vol. 245, � 1. � P. 47�49. doi: 10.1016/0165-7992(90)90024-e.

12. Abraham, S. K. Role of chlorophyllin as an in vivo anticlastogen: protection against gamma-radiation and chemical clastogens / S. K. Abraham, L. Sarma, P. C. Kesavan // Mutation research. � 1994. � Vol. 322, � 3. � P. 209�212. doi: 10.1016/0165-1218(94)90008-6.

13. Morales-Ramirez, P. In vivo effect of chlorophyllin on gamma-ray-induced sister chromatid exchange in murine bone marrow cells / P. Morales-Ramirez, M. C. Garcia-Rodriguez // Mutation research. � 1994. � Vol. 320, � 4. � P. 329�334. doi: 10.1016/0165-1218(94)90085-x.

14. Morales-Ramirez, P. In vivo radioprotective effect of chlorophyllin on sister chromatid exchange induction in murine spermatogonial cells // P. Morales-Ramirez, M. T. Mendiola-Cruz // Mutation research. � 1995. � Vol. 344, � 1-2. � P. 73�78. doi: 10.1016/0165-1218(95)90041-1.

15. Kumar, S. S. Effect of chlorophyllin against oxidative stress in splenic lymphocytes in vitro and in vivo / S. S. Kumar, B. Shankar, K. B. Sainis // Biochimica et biophysica acta. � 2004. � Vol. 1672, � 2. � P. 100�111. doi: 10.1016/j.bbagen.2004.03.002.

16. Ozcan, M. Protective Effects of Antioxidant Chlorophyllin in Chemically Induced Breast Cancer Model In vivo / M. Ozcan, D. Aydemir, M. Bacanli, H. G. Anlar, N. N. Ulusu, Y. Aksoy // Biological trace element research. � 2021. � Vol. 199, � 12. � P. 4475�4488. doi: 10.1007/s12011-021-02585-6.

17. Gomes, B. B. Bioavailability of dietary sodium copper chlorophyllin and its effect on antioxidant defence parameters of Wistar rats / B. B. Gomes, S. B. Barros, E. R. Andrade-Wartha, A. M. Silva, V. V. Silvab, U. M. Lanfer-Marquez // Journal of the science of food and agriculture. � 2009. � Vol. 89. � P. 2003�2009. doi: DOI 10.1002/jsfa.3681.

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18. Hayes, M. Update on the bioavailability and chemopreventative mechanisms of dietary chlorophyll derivatives / M. Hayes, M. G. Ferruzzi // Nutrition research. � 2020. � Vol. 81. � P. 19�37. doi: 10.1016/j.nutres.2020.06.010.

19. Sciuti, L. F. Picosecond dynamic of aqueous sodium-copper chlorophyllin solution: An excited state absorption study / L. F. Sciuti, L. H. Z. Cocca, A. R. L. Caires, P. J. Goncalves, L. de Boni // Chemical Physics Letters. � 2018. � Vol. 706. � P. 652�657. doi: https://doi.org/10.1016/j.cplett.2018.07.016.

20. Kuzin, �. �. Structural and metabolic theory in radiobiology./ �. �. Kuzin // Moscow: Scince, � 1986. � 282 p.

21. Ivanova, I. P. Fenton�s Reaction Induced Chemiluminescence Is Mathematical Modeling of the Process; Characteristics, Parameters and Application Conditions for Biomedical Studies / I. P. Ivanova, S. V. Trofimova, I. �. Piskarev // Modern Technologies in Medicine. � 2014. � Vol. 6, � 4. � P. 14�25.

22. Romodin, L. �. Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 1 / L. �. Romodin // ActaNaturae. � 2021. � Vol. 13, � 3. � P.: 90�100. doi: 10.32607/actanaturae.10912.

23. Sharov, V. S. Assessment of the C-525 laser dye as a chemiluminescence sensitizer for lipid peroxidation in biological membranes: a comparison with chlorophyll-a / V. S. Sharov, K. Briviba, H. Sies // Free radical biology & medicine. � 1996. � Vol. 21, � 6. � P. 833�843.

24. Vlasova, I. I. Peroxidase Activity of Human Hemoproteins: Keeping the Fire under Control / I. I. Vlasova // Molecules. � 2018. � Vol. 23, � 10. � P. 2561. doi: https://doi.org/10.3390/molecules23102561.

25. Yao, Y. The effect of radio frequency heating on the inactivation and structure of horseradish peroxidase / Y. Yao, B. Zhang, H. Pang, Y. Wang, H. Fu, X. Chen // Food chemistry. � 2023. � Vol. 398. � P. 133875. doi: 10.1016/j.foodchem.2022.133875.

26. Sun, F. HRG-9 homologues regulate haem trafficking from haem-enriched compartments / F. Sun, Z. Zhao, M. M. Willoughby, S. Shen, Y. Zhou, Y. Shao, J. Kang, Y. Chen, M. Chen, X. Yuan, I. Hamza, A. R. Reddi, C. Chen // Nature.�2022. � Vol. 610, � 7933. � P.768�774. doi: 10.1038/s41586-022-05347-z.

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All authors have made an equivalent contribution to the preparation of the publication.

The authors declare that there is no conflict of interest.

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К ВОПРОСУ О ПРИМЕНЕНИИ ХЛОРОФИЛЛИНА В КАЧЕСТВЕ РАДИОЗАЩИТНОГО СРЕДСТВА Текст научной статьи по специальности «Ветеринарные науки»

ON THE ISSUE OF THE USE OF CHLOROPHYLLIN AS A RADIOPROTECTIVE AGENT

Текст научной работы на тему «К ВОПРОСУ О ПРИМЕНЕНИИ ХЛОРОФИЛЛИНА В КАЧЕСТВЕ РАДИОЗАЩИТНОГО СРЕДСТВА»