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© Korol D., Kindij D., Korol M., Kozak R., Skubij I.
UDC 616.314-76-77-085.46:611.018
Korol D., Kindij D., Korol M., Kozak R., Skubij I.
HISTOLOGICAL RESEARCH RESULTS
OF SAMARIUM-COBALT MAGNETS SAMPLES
Higher State Educational Establishment of Ukraine «Ukrainian Medical Stomatological Academy» (Poltava)
korolmd@mail.ru
This paper is a part of comprehensive initiative research project of the dental departments of the Higher State Educational Establishment of Ukraine «Ukrainian Medical Stomatological Academy»: «Improvement of orthopedic prevention methods and treatment of secondary edentulism, pathological abrasion, lesions of the periodontal tissues and TMJ diseases in adults on the background of somatic pathology» (state registration number 0111U004872).
Introduction. The need of population in complete removable dentures manufacturing is increasing while taking into account patients who do not use designs made for them. According to WHO data this group comprises from 20% to 26% of the total number of manufactured complete removable dentures. In 52% of such cases the patients complain about unsatisfactory prostheses fixation [2,3].
The problem of dentures fixing on toothless jaws has more than two centuries-old history. But it can't be considered completely solved. The new fixation methods of complete removable dentures are being developed continuously. The use of magnets for the dentures fixation has long been known [4,5,10].
Purposeful application of fixation methods with magnetic adhesion usage enables to achieve required prostheses resistance in orthopedic treatment of patients with teeth loss [6,7,8]. In 50-60 years of the last century magnetic alloys were proposed to apply for improvement of prostheses fixation, but the small co-ercitive power and necessity for frequent magnetization were their disadvantages [1,9].
The aim of the study was to conduct the histologi-cal research of samarium-cobalt magnets samples to define the biocompatibility with the oral cavity tissues when fixing dentures.
Materials and methods. The magnets of samarium-cobalt type (Sm2Co17 XGS30) were the objects of the study included in sanitary-chemical and medico-biological researches.
The experiment was conducted on 10 white laboratory rats weighing 150-200 g. Under general anesthesia, in aseptic conditions the experimental samples of polymagnets were implanted subcutaneously to the dorsal area of the rats. Animals were given the overdose of ether on 7, 14 and 30 days after surgery. Sections with the thickness of 10-15 which were stained with hematoxylin and eosin were made for morphological analysis after standard histological processing (fixation in 10% formalin solution, dehydration in increasing concentrations of ethanol, pouring in paraffin) of
connective-tissue capsule that has formed around the implanted material.
The animal welfare and experiments were carried out in accordance with the provisions of «European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes» (Strasbourg, 1985), «General ethical principles of animal experimentation» adopted by the First National Congress on Bioethics (Kyiv, 2001).
The study results and their discussion. Mac-roscopically all samples around the connective tissue did not differ in color and structure from the tissues far from the site of implantation. Connective tissue capsule was easily detachable from the experimental samples through their smooth surface.
In 7 days after surgery the immature connective tissue capsule around the implanted sample was observed. The inner side of the capsule, which is in direct contact with the polymagnets samples was characterized by round cell infiltration (Fig. 1).
The main cellular elements of the capsule were leukocyte and lymphoid elements as well as macrophages, which were presented in moderate quantity. On the inside of the capsule the fibrin clots were revealed. The young forms of fibroblasts actively producing collagen began to appear at this stage of research. The micro-vasculature response to the experimental sample implantation was negligible, the single vessels of small diameter with normal microcirculation were observed.
In 14 days after surgery the mature and formed enough connective tissue capsule around the experimental sample was revealed (Fig. 2).
Fig. 1. Histological pattern around the implanted sample in 7 days after surgery. Hematoxylin and eosin X100 stain.
Fig. 2. Histological pattern around the implanted sample in 14 days after surgery. Hematoxylin and eosin X100 stain.
The main cellular elements of the capsule were fusiform fibroblasts that were in the bundles plane of mature collagenous fibers arranged along the implanted sample. It should be noted that there is little macrophage infiltration caused by protective organism response to the foreign body implantation. The connective tissue capsule contained single leukocytes and lymphoid elements on the separate sites. The microcirculatory response was negligible, as in the previous investigation period and was characterized by normal microcirculation of single blood vessels.
The thin and mature connective tissue capsule consisting of mature collagen fibers bundles oriented along the implanted sample and fusiform fibroblasts between them was observed in 30 days around the implanted sample (Fig. 3).
The little round cell infiltration, mainly of macrophage elements was revealed on the separate sites of connective tissue capsules. The intensity of the micro-circulatory response was the same as in the previous experimental periods.
Conclusion. Thus, the implantation of experimental samples to the organisms of experimental animals
Fig. 3. Histological pattern around the implanted sample in 30 days after surgery. Hematoxylin and eosin X100 stain.
induced protective-compensatory cellular response of aseptic inflammation type, which was the normal physiological response of the organism to the foreign body presence. According to the morphological characteristics the signs of degenerative changes, tumors and tissue necrosis of connective tissue capsule and surrounding connective tissue around all implanted samples were not observed. Considering the low intensity of the connective tissue cellular response to the implantation of the experimental samples it can be concluded that the experimental samples are biocompatible with the tissues of experimental animals and may be widely used in medical practice after clinical investigations.
Conducted histological studies showed that the experimental polymagnets samples are biocompatible with the tissues of experimental animals and cause negligible reaction according to aseptic inflammation type in the site of their implantation.
Prospects for further research. The experimental study of the effects of samarium-cobalt magnets on the tooth tissues, namely the enamel and dentin with removable and fixed dental prosthetics are planned in future.
References
1. Джамус Белал Использование магнитных сил в ортодонтии: автореф. дис. на соискание научн. степени. канд. мед. наук: спец. 14.00.21-стоматология / Белал Джамус. - Кишинэу, 2004. - 20 с.
2. Згонник О.С. Нуждаемость в полном съемном протезировании населения города Полтавы и области / О.С. Згонник // Актуальш проблеми сучасноТ медицини: Вюник Укр. мед. стомат. акад. - 2003. - Т. З. - Вип. 2 (6). - С. 24-26.
3. Король М.Д. Застосування самарм-кобальтових магнтв у зшмному та незшмному зубному протезуванш. Огляд л^ератури / М.Д. Король, Д.М. Король, Н.В. Головко [та Ы.] // Новини стоматологи. - 2008. - № 1 (54). - С. 53-55.
4. Летягина Р.А. Применение внутрикорневых фиксирующих устройств съемных зубных протезов / Р.А. Летягина, Ж.С. Бяко-ва, А.Г. Рогожников, Е.В. Летягин // Зубной техник. - 2001. - № 2. - С. 40.
5. Марков Б.П. Фиксация протезов на беззубых челюстях / Б.П. Марков // Стоматологический вестник. - 2002. - № 2. - С. 44-55.
6. Ортопедическая стоматология: [руководство для врачей, студ. вузов и мед. училищ] / Н.Г. Аболмасов, Н.Н. Аболмасов, В.А. Бычков, А. Аль-Хаким. - М.: МЕД пресс-информ, 2002. - 576 с.
7. Патент № 2188603 RU МПК A61C13/235 «Устройство для магнитной фиксации съёмного зубного протеза» / С.В. Казаков, Г.Е. Кирко, И.М. Кирко, С.Г. Конюхова, А.Г. Рогожников. - Пермская государственная медицинская академия (RU). - № 2000109001/14; Заявл. 10.04.2000; Опубл. 10.09.2002; Бюл. № 36.
8. Рендо Б. Магнитная балочная конструкция от клыка до клыка: трамплин для перехода к несъемному протезированию на имплантатах / Б. Рендо, Р. Кальвани, Ф. Соллечито, С. Саба, В. Ток, Е. Финадеева // Новое в стоматологии. - 1997. - № 9. - С. 7-24.
9. Самарий-кобальтовый магнит - концентратор / www.imp.uran.ru.
10. Свирин Б.В. Получение функционального слепка с верхней и нижней челюстей после полной утраты зубов, обусловленной заболеваниями пародонта / Б.В. Свирин // Зубной техник. - 2003. - № 6. - С. 18.
УДК 616.314-76-77-085.46:611.018
РЕЗУЛЬТАТИ Г1СТОЛОГ1ЧНИХ ДОСЛ1ДЖЕНЬ ЗРАЗК1В САМАР1Й-КОБАЛЬТОВИХ МАГН1Т1В
Король Д., Киндiй Д., Король М., Козак Р., Скубш I.
Резюме. Метою дослiдження було гiстологiчне вивчення зразкiв самарiй-кобальтових магнтв з метою бiосумiсностi з тканинами порожнини рота при фксацм зымних протезiв. Об'ектами дослiдження служили зразки магнтв типу самарйкобальт (Sm2co17 Xgs30), якi пiдцавалися санiтарно-хiмiчним i медико-бiологiчним дослiдженням.
Експеримент проводився на 10 бтих лабораторних щурах, вагою 150-200 г Пщ наркозом, в умовах асептики, щурам субкутально в област спини iмплантували дослщы зразки полiмагнiту. Тварин виводили з експерименту шляхом передозування ефiром на 7, 14 i 30 добу пюля операцiI.Для морфологiчного аналiзу пюля стандартно! гiстологiчноI обробки сполучнотканинно! капсули, яка сформувалася довкола iмплантованого матерiалу, були виготовленi зрiзи товщиною 10-15 мкм, як забарвлювали гематоксилiном i еозином.
Проведенi гiстологiчнi дослiдження показали, що дослiднi зразки полiмагнiтiв е бiосумiсними з тканинами експериментальних тварин i викликають незначну запальну реакцю за типом асептичного запалення у мюц !х iмплантацiI.
Ключовi слова: самарiй-кобальтовi магнiти, iмплантацiя, гiстологiчнi доотдження.
УДК 616.314-76-77-085.46:611.018
РЕЗУЛЬТАТЫ ГИСТОЛОГИЧЕСКИХ ИССЛЕДОВАНИЙ ОБРАЗЦОВ САМАРИЙ-КОБАЛЬТОВЫХ МАГНИТОВ
Король Д., Киндий Д., Король М., Козак Р., Скубий И.
Резюме. Целью исследования явилось гистологическое изучение образцов самарий-кобальтовых магнитов с целью биосовместимости с тканями полости рта при фиксации съемных протезов. Объектами исследования служили образцы магнитов типа самарий-кобальт (Sm2Co17 XGS30), которые поддавались са-нитарно-химическим и медико-биологическим исследованиям.
Эксперимент проводился на 10 белых лабораторных крысах, весом 150-200 г. Под наркозом, в условиях асептики, крысам субкутально в области спины имплантировали опытные образцы полимагнита. Животных выводили из эксперимента путем передозировки эфиром на 7, 14 и 30 сутки после операции. Для морфологического анализа после стандартной гистологической обработки соединительнотканной капсулы, которая сформировалась вокруг имплантированного материала, были изготовлены срезы толщиной 10-15 мкм, которые окрашивали гематоксилином и эозином.
Проведенные гистологические исследования показали, что опытные образцы полимагнитов являются биосовместимыми с тканями экспериментальных животных и вызывают незначительную воспалительную реакцию по типу асептического воспаления в месте их имплантации.
Ключевые слова: самарий-кобальтовые магниты, имплантация, гистологические исследования.
UDC 616.314-76-77-085.46:611.018
HISTOLOGICAL RESEARCH RESULTS OF SAMARIUM-COBALT MAGNETS SAMPLES
Korol D., Kindij D., Korol M., Kozak R., Skubij I.
Abstract. The problem of dentures fixing on toothless jaws has more than two centuries-old history. But it can't be considered completely solved. The new fixation methods of complete removable dentures are being developed continuously. The use of magnets for the dentures fixation has long been known.
Purposeful application of fixation methods with magnetic adhesion usage enables to achieve required prostheses resistance in orthopedic treatment of patients with teeth loss. In 50-60 years of the last century magnetic alloys were proposed to apply for improvement of prostheses fixation, but the small coercitive power and necessity for frequent magnetization were their disadvantages.
The aim of the study was the histological investigation of samarium-cobalt magnets samples on biocompatibility with the oral cavity tissues when fixing dentures. The magnet samples of samarium-cobalt type (Sm2Co17 XGS30) included to sanitary-chemical and medico-biological researches were the objects of the study.
The experiment was conducted on 10 white laboratory rats weighing 150-200 g. Under general anesthesia, in aseptic conditions the experimental samples of polymagnets were implanted subcutaneously to the dorsal area of the rats. Animals were given the overdose of ether on 7, 14 and 30 days after surgery. Sections with the thickness of 10-15 |im, which were stained with hematoxylin and eosin were made for morphological analysis after standard histological processing of connective tissue capsule that had formed around the implanted material.
Macroscopically all samples around the connective tissue did not differ in color and structure from the tissues far from the site of implantation. Connective tissue capsule was easily detachable from the experimental samples through their smooth surface.
Thus, the implantation of experimental samples to the organisms of experimental animals induced protective-compensatory cellular response of aseptic inflammation type, which was the normal physiological response of the organism to the foreign body presence. According to the morphological characteristics the signs of degenerative
changes, tumors and tissue necrosis of connective tissue capsule and surrounding connective tissue around all implanted samples were not observed.
Considering the low intensity of the connective tissue cellular response to the implantation of the experimental samples it can be concluded that the experimental samples are biocompatible with the tissues of experimental animals and may be widely used in medical practice after clinical investigations.
Conducted histological studies showed that the experimental polymagnets samples are biocompatible with the tissues of experimental animals and cause negligible reaction according to aseptic inflammation type in the site of their implantation.
Keywords: samarium-cobalt magnets, implants, histological examinations.
Рецензент - проф. Ткаченко I. М.
Стаття надшшла 02.12.2016 року
